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Studies with nonradioisotopic sodium chromate
Author(s) -
Heaton W. A. L.,
Keegan T.,
Hanbury C. M.,
Holme S.,
Pleban P.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29890020444.x
Subject(s) - red cell , isotopes of chromium , hematocrit , chemistry , albumin , red blood cell , volume (thermodynamics) , chromatography , chromium , sodium , nuclear chemistry , biochemistry , medicine , physics , organic chemistry , quantum mechanics
A recently developed nonradioisotopic 52 Cr technique was used to measure either red cell volume or posttransfusion recovery of stored red cells. The experimental method uses Zeeman electrothermal atomic absorption spectrophotometry to measure red cell chromium. Results from the 52 Cr method were compared with those from 51 Cr single‐label and 125 I‐albumin/ 51 Cr double‐label procedures using 49‐day AS‐1 red cell concentrates drawn and prepared according to standard procedures. In the first group of five donors, red cell volume was estimated concurrently with both 52 Cr‐labeled fresh red cells and 125 I‐albumin. The latter measured plasma volume from which red cell volume was estimated on the basis of the hematocrit ( 125 I red cell volume). 51 Cr‐labeled stored red cells were transfused to measure posttransfusion recoveries. The correlation between 52 Cr and 125 I red cell volumes was significant (r = 0.68, p < 0.01), and, in this group, the differences were not significant (p > 0.05). Twenty‐four‐hour posttransfusion recoveries of 51 Cr‐labeled stored red cells averaged 66 ± 5 percent when measured with the 125 I/ 51 Cr technique and 69 ± 8 percent when measured with the 52 Cr/ 51 Cr method. In the second group of five donors, red cell volume was estimated by the 125 I‐albumin technique, and the posttransfusion recovery of stored red cells was quantitated by 51 Cr‐ and 52 Cr‐labeled stored cells simultaneously. In this group, posttransfusion recoveries with 125 I/ 51 Cr averaged 73 ± 7 percent; with 125 I/ 52 Cr, they averaged 75 ± 10 percent. Using the single‐label method of calculation, recoveries averaged 76 ± 7 and 75 ± 10 percent for the 51 Cr and 52 Cr methods, respectively. Calculated mean posttransfusion recoveries for the total group (n = 10) showed a small but significantly lower (p < 0.05) posttransfusion recovery with the double‐label 125 I/ 51 Cr technique (69 ± 7%) than that with the single‐label 51 Cr method (74 ± 7%). The nonradioisotopic 52 Cr technique provided an acceptable measure of posttransfusion recovery when used in a double‐label method, either for the determination of red cell volume concurrent with 51 Cr‐labeled stored cells or for the labeling of stored cells with an independent measure of red cell volume.