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Evaluation of the polyethylene glycol‐indirect antiglobulin test for routine compatibility testing
Author(s) -
Slater J. L.,
Griswold D. J.,
Wojtyniak L. S.,
Reisling M. J.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29890020440.x
Subject(s) - peg ratio , antibody , polyethylene glycol , medicine , immunology , chemistry , biochemistry , finance , economics
All specimens received in the blood bank over a 5‐month period for crossmatch or group and screen requests were tested in parallel by a polyethylene glycol‐indirect antiglobulin test (PEG‐IAT) and a low‐ionic‐strength saline (LISS)‐IAT. The sera of 41 of 1471 patients had reactions, with 50 antibodies being detected. Ten antibodies reacted only on the PEG‐IAT and 14 only by the LISS‐IAT; the remaining 26 antibodies were detected by both methods. Of the antibodies that reacted only by the LISS‐IAT, one (anti‐Jk a ) was considered clinically significant, whereas five of the antibodies that reacted only by the PEG‐IAT (1 anti‐c, 2 ‐Fy a , 1‐Jk b , and 1‐S) were considered significant. Two antibodies of questionable clinical significance were detected only by the PEG‐IAT. In 97 percent of the sera tested, no reaction was detected by either method. The PEG‐IAT is an acceptable technique for routine compatibility testing.