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Evaluation of platelet concentrates prepared from buffy coats and stored in a glucose‐free crystalloid medium
Author(s) -
Bertolini F.,
Rebulla P.,
Riccardi D.,
Cortellaro M.,
Ranzi M. L.,
Sirchia G.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29789369678.x
Subject(s) - platelet , buffy coat , platelet concentrate , medicine , blood product , platelet transfusion , andrology , chemistry , surgery , immunology
Comparison was made between platelet concentrates prepared from pools of buffy coats removed from standard blood donations and stored in a glucose‐free, commercially available crystalloid solution (BC‐PCs) and standard platelet concentrates prepared from platelet‐rich plasma (PRP‐PCs). Platelet yield in BC‐PCs and PRP‐PCs was 59 and 75 percent of donated platelets, respectively. The number of total white cells in 1 BC‐PC unit, prepared from a pool of 7 buffy coats, was 21 × 10 6 , i.e., 50 times lower than that of 7 units of PRP‐PCs. The in vitro values of adequate platelet quality were maintained for 10 days in BC‐PCs stored in 1000‐mL polyolefin bags. Prolonged bleeding times were reduced or corrected in three of three thrombocytopenic leukemic patients evaluated before and after transfusion of stored BC‐PCs. Pretransfusion and 1‐ and 24‐hour posttransfusion median platelet counts in 57 leukemic recipients during 4 months of routine transfusion of BC‐PCs (n = 93) were 14,35, and 27 × 10 9 per L, while those of PRP‐PCs (n = 246) were 13, 37, and 31 × 10 9 per L, respectively. No reactions to BC‐PCs were reported, but a 1.3 percent rate of reaction to PRP‐PC transfusions was reported. This study indicates that BC‐PCs are a good alternative to PRP‐PCs for platelet support of thrombocytopenic patients. TRANSFUSION 1989;29:605–609.