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Response to platelet‐activating factor in human platelets stored and aged in plasma. Decrease in aggregation, phosphoinositide turnover, and receptor affinity
Author(s) -
Shukla S. D.,
Morrison W. J.,
Klachko D. M.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29689318453.x
Subject(s) - platelet , platelet activating factor , receptor , chemistry , endocrinology , medicine , clot retraction , platelet activation , platelet aggregation , andrology , biochemistry , biology
Human platelet concentrates were stored in polyolefin bags at 22 to 24° C on a horizontal shaker for up to 8 days. At different intervals, aliquots of platelet‐rich plasma (PRP) were removed aseptically and five variables, i.e., platelet counts, morphology, platelet‐activating factor (PAF)‐stimulated aggregation, phosphoinositide turnover, and [ 3 H]PAF binding to platelet receptors, were studied. The number of platelets did not change during the 8 days of storage. Scanning electron microscopy of the platelets revealed a gradual morphologic change from biconcave flat discs to irregular, crenated forms. The PAF‐induced aggregation of platelets declined with time of storage. A decrease to 50 percent of the Day 1 aggregatory response to PAF was evident on Day 2, and there was a further decline to about 20 percent by Day 6. Similarly, PAF receptor‐coupled phosphoinositide turnover, as monitored by 32 P incorporation into individual phosphoinositides, decreased dramatically with storage. After 2 to 3 days of storage, the phosphoinositide turnover was reduced to 50 percent of the original response, and it continued to decline to about 25 percent of original response by Day 5 or 6. The binding of [ 3 H]PAF to washed human platelets indicated subtle changes between Days 2 and 4, which became more noticeable by Day 6. These results have raised the possibility of changes in the number of the receptors and/or their affinity for the ligand during storage. We conclude that although the number of platelets was maintained during storage for 8 days, a general deterioration of their responses to PAF occurred at the levels of cell surface receptor, transmembrane signaling (phosphoinositide turnover), and response (aggregation).