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Preparation of white cell‐depleted red cells for 42‐day storage using an integral in‐line filter
Author(s) -
Davey R. J.,
Carmen R. A.,
Simon T. L.,
Nelson E. J.,
Leng B. S.,
Chong C.,
Garcez R. B.,
Sohmer P. R.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29689318446.x
Subject(s) - hemolysis , red cell , platelet , red blood cell , zoology , chemistry , chromatography , cellulose , andrology , medicine , immunology , biochemistry , biology
A new blood pack system for the preparation of white cell‐depleted red cells was studied. The system is a modified additive‐solution quadruple‐unit blood pack that incorporates a cellulose‐acetate fiber depth filter in‐line between the AS‐3 additive bag and the CP2D collection bag. Mean ± SD white cell removal from 156 units processed under standard production conditions was 97.7 ± 2.7 percent; residual white cells were 1.1 ± 1.0 × 10 8 per unit. Red cell loss was 10.0 ± 1.0 percent (n = 43). Mean platelet removal was 80.9 percent from units from which platelet concentrates were not prepared (n = 47). Microaggregates did not form during storage, and hemolysis of filtered red cells was lower than that of unfiltered controls. Filtered AS‐3 red cells stored for 42 days had a 51 Cr survival of 80.1 ± 5.7 percent (mean ± SD) as compared with 78.9 ± 6.2 percent for unfiltered controls (n = 17). This in‐line filter system provides white cell‐depleted, microaggregate‐free red cells that can be stored for 42 days.