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Suitability of liquid‐stored donor platelets in platelet compatibility testing
Author(s) -
Kiss J. E.,
Salamon D. J.,
Wilson J.,
Ramsey G.,
Duquesnoy R. J.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29589284139.x
Subject(s) - platelet , apheresis , platelet transfusion , immunology , human leukocyte antigen , chemistry , plateletpheresis , antigen , leukoreduction , platelet concentrate , andrology , medicine
Current platelet crossmatch procedures to select compatible donors for alloimmunized thrombocytopenic patients are hampered by the lack of a convenient platelet storage method. This study examined the feasibility of using washed apheresis donor platelets stored for up to 1 year in a modified Hank's buffer solution at 4° C as crossmatch reagents in an indirect IgG‐enzyme immunoassay. Pooled and monospecific HLA and Pl A1 antisera were used to determine the antigenic reactivity of donor platelets in relation to duration of storage. There were no significant differences between mean HLA and Pl A1 antigen expression in fresh and stored platelets. HLA reactivity was detected on 12 of 13 donor platelet samples stored for 3 to 9 months and on 14 of 17 platelets stored for 12 to 14 months. Pl A1 reactivity was maintained at 12 to 14 months for all 12 donor platelet samples tested. In addition, incompatibility remained in 23 of 24 paired fresh and stored platelet crossmatches using individual alloimmunized patient plasmas. These data indicate that both HLA and platelet‐specific Pl A1 antigen reactivity can be maintained adequately in liquid storage at 4° C for up to 1 year. The availability of a convenient platelet storage method should facilitate the general application of platelet crossmatching procedures for alloimmunized patients.