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The detection of an antibody against IgA allotype A2M 2 and a study of the Am genetic marker among the Han Chinese population
Author(s) -
Wu G.,
Liu D.,
Fang G.,
Hu W.,
Jia H.,
Zhang Q.,
Fukada K.,
Yoshimura K.,
Saji H.,
Lee T. D.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29489242801.x
Subject(s) - allotype , ouchterlony double immunodiffusion , hemagglutination assay , population , biology , antibody , hemagglutination , allele , immunology , allele frequency , gene , genetics , medicine , antiserum , environmental health , titer
The serum of a woman was found by the Ouchterlony double‐diffusion and the hemagglutination inhibition (HAI) methods to have immunoglobulin A (IgA) deficiency. Further investigation using the hemagglutination (HA) test with red cells coated with IgA myeloma proteins of different specificities showed that the serum agglutinated only IgA 2 ‐, A2M 1‐, and A2M 2‐coated cells. The patterns of the HAI test with a reference panel confirmed the presence of two specificities. One was anti‐IgA 2 and the other was a rare antibody against the allotype A2M 2. The anti‐A2M 2 was used for population studies. Testing of the Han Chinese population, including family studies, confirms that A2M.1 and A2M.2 have an autosomal dominant mode of inheritance and are controlled by a codominant allele. The distribution of the two Am genetic markers among the Han Chinese population demonstrated A2M.1 with a gene frequency of 0.553 and A2M.2 with a gene frequency of 0.447 (χ 2 = 0.145, 0.80>p>0.70).