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Production of human warm‐reacting red cell monoclonal autoantibodies by Epstein‐Barr virus transformation
Author(s) -
Andrzejewski C.,
Young P. J.,
Goldman J.,
Spitalnik S. L.,
Silberstein L. E.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29389162722.x
Subject(s) - autoantibody , clone (java method) , autoimmune hemolytic anemia , monoclonal antibody , monoclonal , virology , immunology , virus , antibody , serology , red blood cell , biology , hemolytic anemia , microbiology and biotechnology , gene , genetics
Monoclonal antibody technology has been used in both murine and human systems to produce a variety of antibodies that react with the human red cell (RBC). RBC monoclonal autoantibodies have been obtained from animal models of autoimmune hemolytic anemia (AIHA), but to date no warm‐reactive monoclonal autoantibodies have been generated from human B cells. Using the Epstein‐Barr virus (EBV) transformation method, clones of RBC autoantibodies were generated from two patients with AIHA. These antibodies reacted preferentially at 37° C, agglutinated or bound to a variety of different RBC phenotypes, and were IgM in nature. The serologic reactivity of one clone showed a relative specificity to e+ RBCs that was similar to that seen in the patient's serum. These results are the first to demonstrate that warm‐reactive RBC autoantibodies can be obtained from patients with AIHA using the technique of EBV transformation, and they further substantiate the existence of warm‐reactive IgM RBC autoantibodies in the spectrum of warm AIHA.