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Effect of diltiazem and a prostaglandin derivative (PGB x ) on platelet function during long‐term storage
Author(s) -
Smith D. J.,
Odom D. G.,
Cheney B. A.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29289146835.x
Subject(s) - platelet , diltiazem , chemistry , calcium , endocrinology , medicine , prostaglandin , calcium metabolism , biochemistry , organic chemistry
Calcium is an intermediate messenger between platelet stimuli and platelet response. Published studies have shown that the decreased ability of platelets to control calcium flux during long‐term storage leads to platelet senility. Platelet metabolism might be more efficient during storage if pharmacologic agents that limit calcium movement were incorporated into the platelet concentrate storage solution. This hypothesis was tested by storing platelets with the calcium channel blocker, diltiazem, or with a prostaglandin B 1 , derivative, PGB x . During a 15‐day storage period, platelets incubated with either diltiazem or PGB x showed improved function, as measured by aggregation, as compared to control platelets. The PGB x ‐enhanced platelet function during storage was accompanied by a significant decrease in glucose and an increase in adenosine triphospate concentrations. Platelet function after storage with PGB x improved in spite of significantly lower pH levels of the platelet concentrates at all time points tested. These studies suggest that the maintenance of calcium ion homeostasis during long‐term platelet storage is important to in vitro platelet function even if the Ca 2+ balance is maintained at the expense of pH and the glucose concentrations.