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Use of Indium‐ 111 as a red cell label
Author(s) -
AuBuchon J. P.,
Brightman A.
Publication year - 1989
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1989.29289146833.x
Subject(s) - tropolone , chemistry , chelation , in vivo , red blood cell , isotopes of chromium , red cell , acetylacetone , radiochemistry , chromatography , nuclear medicine , biochemistry , nuclear chemistry , medicine , biology , inorganic chemistry , microbiology and biotechnology , organic chemistry
To select the most promising 111 In chelate for use as a second red cell (RBC) label for comparison of the survival of autologous and allogeneic cells, 49 normal RBC samples were studied in vitro after being labeled with 111 In‐8‐hydroxyquinolinol ( 111 In‐oxine) prepared by three different methods, 111 In‐tropolone, and 111 In‐acetylacetone. Labeling efficiencies reached 99 percent and did not decline when the amount of 111 In used was increased from 1.75 to 50 μCi per ml of RBCs. Storage of labeled RBCs in normal AB plasma at 4, 22, and 37°C for up to 48 hours resulted in a similar rate of loss of the label from the RBCs with all labeling methods. These rates were time‐ and temperature‐dependent and were accurate predictions of the rates found in later in vivo experimentation. Fresh RBCs from 11 subjects were labeled with 111 In chelated with oxine in the presence of the RBCs or chelated with tropolone just prior to the labeling. RBC mass determinations using these autologous RBCs labeled with 111 In accurately reflected the subjects' RBC masses as predicted through standard morphometric formulae. The rate of disappearance of the radionuclide after reinfusion of the autologous RBCs decreased with time. At 24 hours after reinfusion, 89.5 ± 1.29 percent (mean ± SEM) of the 111 In‐tropolone and 87.3 ± 1.25 percent of the 111 In‐oxine continued in circulation. 111 In is a simple and efficient agent for the labeling of RBCs for blood volume determinations and short‐term survivals.