Premium
Noncellular immunoadsorbents for preparing typing sera that recognize high‐frequency red cell antigens
Author(s) -
Gee S. W.,
Lubenko A.
Publication year - 1988
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1988.28689059030.x
Subject(s) - abo blood group system , antigen , antibody , microbiology and biotechnology , typing , chemistry , covalent bond , titration , immunology , biology , genetics , inorganic chemistry , organic chemistry
The feasibility of using salivary ABH substance, covalently coupled to an affinity gel as an immunoadsorbent for anti‐A and ‐B in sera containing antibodies to high‐frequency antigens was investigated. Several examples of anti‐Hy (n = 4), ‐Jr a (n = 3), ‐U (n = 3), ‐Lu b (n = 3), ‐Tj a (n = 8), and ‐Vel (n = 6), plus one anti‐Co a , were treated by batch adsorption with the affinity gel. Titration of the adsorbed sera with ABO‐incompatible cells lacking the relevant high‐frequency antigen, in parallel with ABO‐compatible cells expressing the high‐ frequency antigen, revealed that ABO antibodies could be adsorbed to exhaustion without loss of the sera's activity against cells bearing high‐frequency antigens.