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Enhanced reaction with Vicia graminea lectin and exposed terminal N− acetyl‐D‐glucosaminyl residues on a sample of human red cells with Hb M− Hyde Park
Author(s) -
King M. J.,
Liew Y. W.,
Moores P. P.,
Bird G. W.
Publication year - 1988
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1988.28689059029.x
Subject(s) - terminal (telecommunication) , lectin , sample (material) , vicia , microbiology and biotechnology , biology , chemistry , biochemistry , botany , vicia faba , chromatography , computer science , telecommunications
A sample of polyagglutinable red cells was obtained from a healthy individual (group O, N) possessing a hemoglobin (Hb) variant called Hb M‐Hyde Park. The sialic acid content of the individual's red cells is 90 percent of normal, and his cells are agglutinated by monoclonal but not lectin anti‐Tn, a panel of lectins specific for N‐ acetylgalactosamine (or galactose), and N‐acetylglucosamine. Enhanced agglutination reactions were obtained with Vicia graminea , Ulex europaeus, and human anti‐I and ‐i. Using various enzyme treatments and different methods of labeling cell surface components, two defective cell membrane sites have been identified: one associated with the O‐ linked oligosaccharides on sialoglycoproteins and the other associated with exposed N‐acetylglucosaminyl residues located on membrane components of apparent molecular weights 88,000 to 130,000 and 46,000 to 73,000 (probably the Band 3 and Band 4.5 regions, respectively).