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Quantitation of red cell‐bound immunoglobulin and complement using enzyme‐linked antiglobulin consumption assay
Author(s) -
Kiruba R.,
Han P.
Publication year - 1988
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1988.28689059023.x
Subject(s) - antibody , autoimmune hemolytic anemia , immunology , immune system , chemistry , hemolytic anemia , complement system , immunoglobulin g , red cell , enzyme , coombs test , immunoglobulin m , hemolysis , red blood cell , microbiology and biotechnology , medicine , biology , biochemistry
Various techniques have been described for quantitating IgG or complement (C3) on red cells (RBCs). The techniques either are cumbersome, as the complement consumption test, or use radioactivity. This paper describes an antiglobulin consumption assay using an enzyme‐ linked immunosorbent method that can be used to quantitate IgG, IgM, and C3. With this technique RBCs from normal, healthy donors gave a mean value of 106 ± 60 molecules of IgG per RBC, 4.5 ± 3 molecules of IgM per RBC, and 37 ± 28 molecules of C3 per RBC, respectively. The RBCs of hospital patients, particularly of those with infections or inflammatory conditions, contain increased amounts of nonspecifically bound immunoproteins. The availability of a common method to quantitate RBC‐associated IgG, IgM, and C3 allows easy monitoring or study of the immune mechanism of autoimmune hemolytic anemia.