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Detection of HLA antibodies by platelet crossmatching techniques
Author(s) -
McGrath K.,
Holdsworth R.,
Veale M.,
Bishop J.,
Wolf M.
Publication year - 1988
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1988.28388219145.x
Subject(s) - platelet , radioimmunoassay , antibody , human leukocyte antigen , immunofluorescence , immunology , antiserum , antigen , medicine , indirect immunofluorescence
Thirty‐seven monospecific HLA antibodies directed against all common HLA‐A and ‐B loci and reactive by the microlymphocytotoxicity assay (LCT) were tested against platelets carrying the corresponding antigen by three platelet crossmatch methods, the platelet immunofluorescence test (PIFT), platelet enzyme‐linked immunosorbent assay (P‐ELISA), and platelet radioimmunoassay (P‐RIA). Positive reactions were obtained with the PIFT in 67 percent, the P‐ELISA in 41 percent, and the P‐RIA in 49 percent of 85 cell‐serum pairs. The same cell‐serum combinations gave 49 percent positive reactions in the lymphocyte immunofluorescence test. Three multispecific HLA antisera were positive in nine of nine cell‐serum combinations by all four methods. Thirteen transfusions were given to eight patients with known HLA antibodies. All donor‐recipient pairs were LCT positive, six were PIFT positive, and seven were PIFT negative. Three of seven PIFT‐negative and none of six PIFT‐positive transfusions were successful. Thus, platelet crossmatching is less sensitive than the LCT for the detection of complement‐binding monospecific HLA antibodies. The platelet crossmatch, however, is able to identify some potentially successful HLA‐incompatible donors for patients with multispecific HLA antibodies and limited access to HLA‐ identical donors.