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Evidence of an anti‐A 1 inhibited by EDTA
Author(s) -
Tedrow H.E.,
Zeigler Z.R.
Publication year - 1988
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1988.28288179026.x
Subject(s) - hemolysis , diluent , reagent , chemistry , chelation , cold agglutinin , edetic acid , reactivity (psychology) , lysis , immunology , antibody , chromatography , medicine , biochemistry , nuclear chemistry , pathology , inorganic chemistry , alternative medicine
EDTA is added to some commercial A and B cells as a means of preventing hemolysis in serum testing. Unexpected results in serum testing have been reported with EDTA‐dependent agglutinins, but not with agglutinins inhibited by EDTA. A serum sample was found to contain an anti‐A1 undetected by serum testing, but resulting in incompatible crossmatches. Studies indicated that reactivity was inhibited by the EDTA present in the diluent of the A reagent red cells.