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Hybrid sialoglycoprotein content of St(a+) red cells
Author(s) -
Rearden A.
Publication year - 1988
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1988.28288179013.x
Subject(s) - sialoglycoprotein , content (measure theory) , medicine , immunology , mathematics , antibody , mathematical analysis
St(a+) red cells contain a ficin‐resistant hybrid sialoglycoprotein (SGP) consisting of the amino terminus of Ss sialoglycoprotein (Ss SGP) and the carboxyl terminus of MN sialoglycoprotein (MN SGP). Ficin‐ modified St(a+) but not St(a−) red cells were agglutinated by monoclonal antibodies NN5 (anti‐N) and 31 (detecting a sialic acid‐ dependent determinant on the SGPs). SDS‐polyacrylamide gel electrophoresis showed three periodic acid‐Schiff‐staining protein bands in ficin‐modified St(a+) but not St(a−) red cell‐membranes; these bands bound monoclonal antibodies NN5 and 31 on Western blots. Using Scatchard analysis of 125I‐NN5 IgG binding to M+N− red cells, estimates were obtained of 25,000 Ss SGP molecules per red cell per s allele, 33,000 Ss SGP molecules per red cell per S allele, and 75,000 hybrid SGP molecules per red cell per hybrid allele. These results are consistent with the expression of the hybrid SGP gene at a level intermediate between the MN SGP gene and the Ss SGP gene. Monoclonal antibodies NN5 and 31 may be useful in screening ficin‐treated red cells for hybrid SGP variants.