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Liquid storage at 4 ° C of previously frozen red cells
Author(s) -
Moore G.L.,
Ledford M.E.,
Mathewson P.J.,
Hankins D.J.
Publication year - 1987
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1987.27688071704.x
Subject(s) - preservative , glycerol , mannitol , blood preservation , chemistry , lysis , adenosine triphosphate , red cell , food science , biochemistry , chromatography , andrology , medicine
Fresh human blood was collected in citrate‐phosphate‐dextrose, frozen by a high‐glycerol technique, and stored at −80 ° C. The red cells were thawed, deglycerolized, and resuspended in a final wash solution, ADSOL (Fenwal Laboratories), or an additive solution (AS) containing glucose, adenine, mannitol, and phosphate. The cells were then stored at 4 to 6 ° C for 21 days and assayed weekly for adenosine triphosphate and 2,3 diphosphoglycerate, pH, glucose use, and lysis. AS and, to a lesser extent, ADSOL produced metabolic profiles similar to or better than profiles of cells not frozen and stored in commercially available additive solutions. AS offers a potential post‐ thaw preservative solution for red cells that would greatly increase the flexibility and reduce the expense of using frozen blood. A sterile post‐thaw storage capability will make the stockpiling of frozen red cells a practical concept for both military and civilian blood banks.