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Radiolabeled red cell viability
Author(s) -
Marcus C.S.,
Myhre B.A.,
Angulo M.C.,
Salk R.D.,
Essex C.E.,
Demianew S.H.
Publication year - 1987
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1987.27587320536.x
Subject(s) - viability assay , chemistry , red cell , albumin , red blood cell , chromatography , isotopes of chromium , hydroxyethyl starch , andrology , radiochemistry , biochemistry , cell , medicine , nuclear chemistry
The simultaneous determination of autologous 99m Tc red cell (RBC) and 51 Cr RBC viability at 24 hours was measured in 19 normal volunteers whose RBCs had been stored in additive media (Nutracel) for 42 or 49 days. The ratio of the 51 >Cr: 99m Tc value was 1.23. In this experiment we also calculated 51 Cr RBC viability by both the single‐isotope method (extrapolation) and the double‐isotope method (using 125 I human serum albumin for an independent plasma volume) in the same volunteers. The corresponding viability values were not significantly different. The simultaneous determination of autologous 111In‐oxine RBC and 51 Cr RBC viability at 24 hours was measured in 19 other normal volunteers whose RBCs had been stored in citrate‐phosphate‐dextrose‐adenine (CPDA‐1) for 1 or 15 days. The ratio of the 51Cr:111In value was 1.1. Use of these 24‐hour viability ratios as conversion factors permits direct comparison of 99m Tc or 111 In RBC viability with a 51 Cr standard, and therefore expands the application of these newer RBC radiolabels.