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Comparison of a commercial hexadimethrine bromide method and low‐ionic‐ strength solution for antibody detection with special reference to anti‐ K
Author(s) -
Letendre P. L.,
Williams M. A.,
Ferguson D. J.
Publication year - 1987
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1987.27287150184.x
Subject(s) - bromide , ionic strength , ionic bonding , chemistry , medicine , inorganic chemistry , ion , organic chemistry , aqueous solution
The sensitivities of manual low‐ionic hexadimethrine bromide (Polybrene, LIP) and low‐ionic Polybrene indirect antiglobulin tests (LIPAT) were compared with those of a manual low‐ionic‐strength indirect antiglobulin test (LISS) by using a commercial Polybrene kit. One hundred antibodies were coded, titrated, and tested in parallel. LIP did not detect 36 antibodies: 31 anti‐K, two anti‐E, two anti‐Fya, and one anti‐Jka. LIPAT did not detect seven anti‐K, two anti‐E, and two anti‐Jka. The combination of LIP and LIPAT did not detect two anti‐ E that were reactive only in a two‐stage enzyme test and seven anti‐K that had titers of 2 or lower by LISS. LISS detected all antibodies except for the two enzyme‐reactive anti‐E. There were no significant differences in the titers of 63 percent of the antibodies studied. For 54 percent of the antibodies in the Kell system, LISS produced significantly higher titers; for 25 percent of antibodies in the Rh system, LIP did so. The poor sensitivity of the Polybrene kit for anti‐ K makes it unsuitable as a primary method for antibody screening.