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Comparison of a manual hexadimethrine bromide‐antiglobulin test with saline‐ and albumin‐antiglobulin tests for pretransfusion testing
Author(s) -
Anderson G.,
Mintz P. D.
Publication year - 1987
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1987.27287150183.x
Subject(s) - medicine , abo blood group system , antibody , coombs test , antigen , isoantibodies , albumin , immunology
A prospective double‐blind study compared a manual hexadimethrine bromide (Polybrene) antiglobulin antibody detection test (P‐AHG) and crossmatch with the albumin‐antiglobulin antibody detection test and saline‐antiglobulin crossmatch routinely used in our laboratory. A total of 10,084 pretransfusion blood samples from approximately 6000 patients were tested. The P‐AHG method detected 153 of 157 alloantibodies for which antigen‐negative, crossmatch‐compatible blood is routinely provided. All four antibodies not detected were anti‐K. The routine techniques detected 147 of the 157 alloantibodies. The P‐ AHG method detected only 36 percent of the alloantibodies for which crossmatch‐compatible blood is routinely provided without determination of the antigen status of the donor unit's red cells (e.g., anti‐Lea), whereas the routine method detected 91 percent of such antibodies. Eighty‐six percent of the 189 alloantibodies detected by the Polybrene technique were found before the addition of antiglobulin. The manual Polybrene test is a rapid and sensitive technique; it may be used without an antiglobulin phase as a routine crossmatch procedure when accompanied by a sensitive antibody detection test that includes antiglobulin and an additional test to ensure ABO compatibility.