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A second discriminator for biological false positive results in enzyme‐ linked immunosorbent assays for antibodies to human immunodeficiency virus (HTLV‐III/LAV)
Author(s) -
WARTICK MARY G.,
MCCARROLL DAVID R.,
WILTBANK THOMAS B.
Publication year - 1987
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1987.27187121452.x
Subject(s) - antigen , virology , antibody , population , western blot , immunology , virus , biology , false positive paradox , lymphocyte , medicine , gene , genetics , environmental health , machine learning , computer science
Two commercially available enzyme‐linked immunosorbent assays (ELISA) were compared in screening a large population of volunteer blood donors. One ELISA utilized the human T‐lymphotropic virus, Type III (HTLV‐III) grown on National Institutes of Health T‐lymphocyte cell line, H‐9, as antigen source; the second used lymphadenopathy associated virus (LAV) grown on Pasteur Institutes' T‐lymphocyte cell line, CEM‐F. Biological false positives (BFP) occurred at a rate of approximately 0.5 percent using each antigen source. However, distinct populations of BFP donors were detected when the two antigen sources were compared. Results indicate that at least two separate sets of antigens are recognized in ELISA by our normal population and result in BFP. Sequential utilization of tests using these distinct sources adds a second discriminator to identification of BFP, with the potential for decreasing the requirement for Western blot analysis.