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Inactivation of human T‐cell lymphotropic virus, type III by heat, chemicals, and irradiation
Author(s) -
QUINNAN GERALD V.,
WELLS MARTHA A.,
WITTEK ALEC E.,
PHELAN MICHAEL A.,
MAYNER RONALD E.,
FEINSTONE STEPHEN,
PURCELL ROBERT H.,
EPSTEIN JAY S.
Publication year - 1986
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1986.26587020131.x
Subject(s) - infectivity , virus , chemistry , virology , virus inactivation , in vitro , ultraviolet light , chromatography , ether , microbiology and biotechnology , biology , biochemistry , organic chemistry , photochemistry
Infectivity of human T‐cell lymphotropic virus, Type III (HTLV‐III) was inactivated by heat more rapidly if in liquid medium than if lyophilized and more rapidly at 60° than 56°C. When HTLV‐III was added to factor VIII suspension, then lyophilized and heated at 60°C for 2 hours or longer there was elimination of 1 × 10 6 in vitro infectious units (IVIU) of virus. Much of the viral inactivation appeared to result from lyophilization. The application of water‐saturated chloroform to the lyophilized material containing virus also resulted in elimination of infectivity. HTLV‐III was efficiently inactivated by formalin, beta‐propiolactone, ethyl ether, detergent, and ultraviolet light plus psoralen. The results are reassuring regarding the potential safety of various biological products.