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The M 1 and Tm antigens require M and N gene‐specified amino acids for expression
Author(s) -
Issitt P. D.,
Wren M. R.,
Moore R. E.,
Roy R. B.
Publication year - 1986
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1986.26587020114.x
Subject(s) - neuraminic acid , antigen , antibody , sialoglycoprotein , amino acid , microbiology and biotechnology , gene , peptide sequence , sequence (biology) , chemistry , glycosylation , biochemistry , biology , glycoprotein , genetics
It is known that the major red ceil sialoglycoprotein (SGP) of M 1 + red cells has the same amino acid sequence as M SGP, whereas that of Tm+ cells has the same sequence as N SGP. M 1 and Tm are serologically demonstrable when a substitution of N ‐acetyl‐D‐glucosamine for N ‐acetyl‐neuraminic acid occurs in one or more of the alkali‐labile tetrasaccharides bound at positions 2, 3, and/or 4 of M or N SGP, respectively. The authors used two serums containing anti‐M and potent anti‐M 1 and two containing anti‐Tm in tests for antibody crossreactivity and cross adsorption and in antibody inhibition studies. The distinct specificities of anti‐M 1 and anti‐Tm, as determined by these studies, show that the M and N gene‐specified amino acids are more important than the glycosylation change in the fine structure of M 1 and Tm antigens.