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Bacterial sepsis secondary to platelet transfusion: an adverse effect of extended storage at room temperature
Author(s) -
BRAINE H.G.,
KICKLER T.S.,
CHARACHE P.,
NESS P. M.,
DAVIS J.,
REICHART C.,
FULLER A.K.
Publication year - 1986
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1986.26486262752.x
Subject(s) - staphylococcus epidermidis , platelet , contamination , sepsis , platelet transfusion , bacteria , microbiology and biotechnology , plateletpheresis , staphylococcus aureus , medicine , food science , biology , immunology , apheresis , ecology , genetics
In a 3‐month period four episodes of bacterial sepsis related to contaminated random donor platelet concentrates were observed. Evaluation incriminated extended (5 or more days) platelet storage. To determine if platelets stored for longer periods were likely to have greater bacterial contamination, platelet concentrates were innoculated with bacteria and daily quantitative bacteriology was performed. In vitro studies using polyolefin bags indicated contamination could occur with a single organism of Staphylococcus epidermidis. By 72 hours, platelet concentrates contained from 10 3 to 10 8 organisms per 0.1 ml depending on the inoculum size. By 6 days all inoculated units contained 10 7 to 10 9 organisms per 0.1 ml.