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Sequestration of serotonin in stored platelets
Author(s) -
Shimizu T.,
Kato K.
Publication year - 1986
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1986.26386209387.x
Subject(s) - platelet , serotonin , chemistry , chromatography , 5 ht receptor , lysis , platelet aggregation , biochemistry , medicine , receptor
A high‐pressure liquid chromatographic procedure was used for the determination of 5‐hydroxytryptamine (serotonin, 5HT) in platelets. The method, which is based on the separation on a reverse‐phase column and measurement of native fluorescence in an acidified mobile phase, had a detection limit of femtomol quantities of 5HT. The mean contents of 5HT extracted from the Triton X‐100‐lysed platelets in random‐donor platelet concentrates (PCs) were 0.39 ± 0.19 μmol per 10 11 platelets (n=5), the value of which was almost equal to 0.40 ± 0.09 μmol per 10 11 platelets (n=15) of platelets prepared by cytapheresis. The fate of platelet 5HT during storage of PCs at 22° C with agitation was investigated for 5 days. Nearly all amounts of 5HT were sequestered within platelets after 5‐day storage. No increased amounts of major metabolites of 5HT, 5‐hydroxytryptophol and 5‐hydroxy‐3‐indoleacetic acid, were detected in plasma. These data suggest that 5HT stored in dense granules of platelets is not metabolized during storage of PCs at 22°C for 5 days.