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A new test useful in identifying red cells with a (δ‐α) hybrid sialoglycoprotein
Author(s) -
VengelenTyler V.,
Mogck N.
Publication year - 1986
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1986.26386209375.x
Subject(s) - sialoglycoproteins , antigen , sialoglycoprotein , chromatography , microbiology and biotechnology , glycophorin , chemistry , biology , immunology , glycoprotein
St(a+) and Dantu+ red cells (RBCs), treated with 0.1 percent ficin solutions, reacted strongly with Vicia graminea lectin (anti‐N vg ). No other RBC sample tested gave these results. This test (ficin‐pretreated RBCs tested with anti‐N vg [FT‐N vg ]) was used to screen the RBCs of 300 Oriental and 100 black donors. Three percent of the Oriental donor samples tested were FT‐N vg +, whereas no FT‐N vg + RBCs were found in the black donor samples tested. The FT‐N vg test is easy to perform and gives selective reactions with antigens associated with (δ‐α) hybrid sialoglycoproteins, such as St a and Dantu. This test can be used for mass screening of RBCs in a search for St(a+) or Dantu+ RBCs. Another application of the FT‐N vg test would be to screen RBCs suspected of having a low‐incidence antigen before using scarce anti‐St a and ‐Dantu reagents.