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Relative specificity of enzyme‐linked immunosorbent assays for antibodies to human T‐cell lymphotrophic virus, type III, and their relationship to Western blotting
Author(s) -
FANG C.T.,
DARR F.,
KLEINMAN S.,
WEHLING R.H.,
DODD R.Y.
Publication year - 1986
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1986.26286152918.x
Subject(s) - antibody , blot , western blot , virology , virus , enzyme , population , microbiology and biotechnology , biology , immunology , medicine , biochemistry , gene , environmental health
A population of 73 donor samples was assembled on the basis of reactive results in routine screening with three different licensed human T‐lymphotrophic virus type III (HTLV‐III) antibody enzyme‐linked immunosorbent assay (ELISA) procedures. The samples were retested by a number of licensed and developmental tests and by Western blot analysis. Our data indicate that nonspecific results are generated by ELISA tests and that many of these reactions appear to be directed against the cell substrate used to grow the virus. These findings suggest that combinations of currently licensed ELISA tests, based upon HTLV‐III grown in H‐9 cells, cannot be used to confirm the specificity of reactive samples.