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The effect of citrate, calcium, and magnesium ions on the potassium movement across the human platelet membrane
Author(s) -
WeisFogh U. S.
Publication year - 1985
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1985.25485273813.x
Subject(s) - chemistry , calcium , potassium , membrane , permeability (electromagnetism) , membrane permeability , ouabain , platelet , magnesium , intracellular , biophysics , sodium citrate , ion transporter , biochemistry , sodium , medicine , biology , organic chemistry , pathology
The intracellular K + concentration in platelets is reduced during storage in citrated plasma. As shown previously, pH has a marked influence on this reduction. The effect of citrate and Ca 2+ and Mg 2+ on the K + permeability of the membrane and on the active K + transport across the membrane was investigated. Platelets were incubated in dialyzed plasma at 37 degrees C, pH 5.5 to 7.9, with and without added Ca 2+ and Mg 2+ and citrate. After 60‐ to 120‐minute incubation, a steady state was reached, and ouabain was added to inhibit the active K + transport. In the pH interval 5.5 to 6.2 citrate, Ca 2+ and Mg 2+ had no influence on either the active K + transport or the K + permeability. Between pH 7.1 and 7.9, Ca 2+ and Mg 2+ decreased the permeability, whereas citrate increased it. At pH 7.9, citrate had a depressing effect on the active transport. These results indicate that a low citrate concentration and a pH below 7 are important for maintaining a high intracellular K + of platelets during storage in plasma.