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The effect of temperature and mode of agitation on the resuspension of platelets during preparation of platelet concentrates
Author(s) -
WELCH M.,
CHAMPION A. B.
Publication year - 1985
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1985.25385219918.x
Subject(s) - platelet , centrifuge , suspension (topology) , platelet aggregation , chemistry , zoology , materials science , biomedical engineering , mathematics , medicine , biology , physics , homotopy , pure mathematics , nuclear physics
A problem arose in a blood bank in which about 10 percent of platelet concentrates (PC) observed at day 1 had a large number of macroscopic aggregates. The aggregates were clumps of platelets that did not go into suspension during preparation of PC. These units were set aside for observation. A maximum of 25 percent were stored on a circular rotator; the remainder were stored on either an elliptical rotator or horizontal shaker. By the end of storage, 6 percent of the units still had a large number of clumps. This percentage was reduced to 2.4 percent, when all clumped units were stored on a circular rotator. The number of clumped units observed at day 1 and at the end of storage were reduced dramatically when the temperature of the laboratory (18–19°C), centrifuge (20°C) and storage environmental chamber (22°C) were increased to 24°C. The units with clumps at day 5 were reduced from 225 (out of 8316) to 25 (out of 7137).