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Posttransfusion purpura
Author(s) -
Dunstan R. A.,
Rosse W. F.
Publication year - 1985
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1985.25385219901.x
Subject(s) - platelet , antibody , medicine , purpura (gastropod) , monoclonal antibody , platelet membrane glycoprotein , immunology , glycoprotein , blood transfusion , microbiology and biotechnology , biology , ecology
A case of posttransfusion purpura is reported in which the laboratory determination of the specificity of the causative antibody was initially confused by the presence in the patient's serum of HLA‐ directed antibodies. The patient was a multiparous 65‐year‐old woman with a previous history of blood transfusion. She developed the typical clinical features of posttransfusion purpura 8 days following the transfusion of 6 units of packed red cells. The patient was P1 A1 negative, and her serum reacted strongly in a radiolabeled monoclonal antibody assay with both P1 A1 ‐positive and ‐negative platelets. Radioimmunoprecipitation demonstrated that the patient's serum contained antibodies both of anti‐P1 A1 and anti‐HLA specificity. Western blotting of normal platelets incubated with the patient's serum verified that the anti‐P1 A1 antibody was directed against platelet membrane glycoprotein III.