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Extended storage of platelets in a new plastic container
Author(s) -
Snyder E. L.,
Ezekowitz M.,
Aster R.,
Murphy S.,
Ferri P.,
Smith E.,
Rzad L.,
Davisson W.,
Pope C.,
Kakaiya R.,
Buchholz D. H.
Publication year - 1985
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1985.25385219899.x
Subject(s) - platelet , plastic bag , chemistry , polyvinyl chloride , plasticizer , platelet concentrate , zoology , andrology , surgery , medicine , materials science , biology , composite material , organic chemistry
A new blood container material (PL 1240 plastic) made of polyvinyl chloride containing a tri(2‐ethylhexyl) trimellitate plasticizer was evaluated in three laboratories. When platelet concentrates (50–60 ml) were stored on a variety of agitators for 7 days at 22 ± 2°C, poststorage pH (mean ± SD) ranged from 7.29 ± 0.05 (6 rpm elliptical rotator) to 6.87 ± 0.8 (70 cycles per minute flatbed agitator). The platelet counts ranged from 1.51 ± 0.12 to 0.95 ± 0.36 × 10 6 per μ|. Morphology scores and hypotonic shock response values of platelets stored 7 days in PL 1240 plastic containers were better than those noted following 3‐day storage of control platelets in PL 146 plastic containers. The percent discharge of lactic dehydrogenase from platelets stored 7 days in PL 1240 plastic containers for 3 days (p < 0.05). Mean platelet recoveries of 44 ± 15 percent (n = 11; 111 Indium) and 39 ± 8 percent (n = 29; 51 Chromium) were seen when autologous platelets were infused following 5‐day storage in PL 1240 plastic bags. Platelet half‐lives of 3.6 ± 0.4 (n = 9) 4.1 ± 0.4 (n = 20) days were reported in the two laboratories which used 51 Cr labeling, while survival values of 7.0 ± 1.0, 2.8 ± 0.8, and 5.4 ± 1.9 days were seen when data from the 111 Indium studies (n = 11) were analyzed using linear, exponential, and multiple hit programs, respectively. Platelets stored for 5 days also were administered to 13 thrombocytopenic oncology patients. In eight instances, a second (control) transfusion of platelets stored 2 to 5 days in PL 732 plastic containers was given to the recipient. There was no difference seen in postinfusion corrected platelet increments (15.6 ± 12.1 × 10 3 /μ| versus 15.2 ± 10.4 × 10 3 /μ| per m 2 /10 11 platelets infused) for cells stored in PL 1240 and PL 732 plastic containers, respectively. Hemostatic efficacy was seen posttransfusion in all four bleeding recipients. We conclude that platelets stored in PL 1240 plastic containers for 5 days are safe and efficacious for use in clinical transfusion practice.