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Extended storage of platelets in a new plastic container
Author(s) -
Grode G.,
Miripol J.,
Garber J.,
Barber T.,
Buchholz D. H.
Publication year - 1985
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1985.25385219898.x
Subject(s) - platelet , platelet concentrate , chemistry , bicarbonate , pco2 , ultrastructure , torr , andrology , chromatography , medicine , anatomy , physics , organic chemistry , thermodynamics
A new polyvinyl chloride container plasticized with tri(2‐ethylhexyl) trimellitate (PL 1240 plastic) was evaluated for use in extended platelet storage. Six leukocyte‐rich platelet concentrates (mean, 0.6 × 10 9 white cells per bag; range, 0.3 to 1.0 × 10 9 per container) were prepared by removing as much of the platelet‐rich plasma from blood as possible. The cells were stored at 22°C on an end‐over‐ end agitator. An average of 1.04 ± 0.19 × 10 11 platelets was recovered, and the mean pH dropped from 7.23 on day 0 to 6.68 by day 5. At the completion of the storage period. PO 2 averaged 80 torr, PCO2 was 35 torr, bicarbonate concentration was 0.5 m M , and lactate concentration 29.5 m M . Thirty‐one additional units of platelet concentrates, not deliberately prepared to be leukocyte‐rich, on day 5 had a pH of 6.75 ± 0.39 (mean platelet yield, 0.97 ± 0.21 × 10 11 ; P O2 and P CO2 averaged 50 and 48 torr, respectively). Following storage, the cells had an average phase microscopic morphology score of 244 (n = 17). Platelets appeared to be preserved well throughout storage when assessed by transmission and scanning electron microscopy. We conclude that platelets can be stored for 5 days in PL 1240 plastic containers with good preservation of pH and cell ultrastructure.