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Separation of large quantities of mononuclear cells from human blood using a blood processor
Author(s) -
Beaujean Françoise,
Gourdin MarieFrançoise,
Farcet JeanPierre,
Forestier Chantal Le,
Bouguet Jacqueline,
Reyes Felix,
Duedari Najib
Publication year - 1985
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1985.25285169210.x
Subject(s) - peripheral blood mononuclear cell , centrifugation , chromatography , blood cell , monoclonal antibody , separator (oil production) , chemistry , antibody , immunology , biology , biochemistry , in vitro , physics , thermodynamics
A blood processor (IBM 2991) was used to separate lymphocytes from large volumes of blood. The procedure included the centrifugation of 200 ml whole blood on a density gradient. The results of this procedure were compared with those obtained with a manual procedure. Mononuclear cell (MNC) viability was preserved well in the two methods. But with the processor, recovery of MNC was better (63.5 +/− 2.5%) than with manual separation (26.5 +/− 4.1%). Monoclonal antibodies were used to identify the various cell subsets in the MNC fractions. No particular cell selection was observed when MNC fractions were obtained by the separator. In conclusion, the use of a cell separator provided an efficient technique for rapid isolation of large quantities of lymphocytes.