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Microtest for red cell typing
Author(s) -
Leong Sau Wa,
Terasaki Paul I.
Publication year - 1985
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1985.25285169209.x
Subject(s) - typing , reagent , red cell , blood typing , antiserum , antibody , chromatography , red blood cell , chemistry , microbiology and biotechnology , medicine , biology , immunology
A simple microtest for red cell (RBC) typing was developed which required 0.002 ml of reagents per test. After mixing RBCs with antibody, the microtray was incubated upside down for 10 to 15 minutes at 37 degrees C, and 10 minutes at room temperature. The trays were read after reinversion and allowing 10 to 15 minutes for the RBCs to settle. Antibodies must be selected that react under these conditions. Two examples each of anti‐A, ‐B, ‐C, ‐D, ‐E, ‐c, ‐M, and ‐N were tested against a panel of 500 individuals. The results were generally concordant with each other and with the conventional tube test results. The procedure is simple to perform and involves minimal costs for reagent antisera.