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Expression of red cell antigens by K562 human leukemia cells before and after induction of hemoglobin synthesis by hemin
Author(s) -
McGinniss Mary H.,
Dean Ann
Publication year - 1985
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1985.25285169197.x
Subject(s) - hemin , glycophorin , antigen , pan t antigens , biology , fetal hemoglobin , k562 cells , microbiology and biotechnology , hemoglobin , leukemia , immunology , fetus , biochemistry , antibody , enzyme , heme , genetics , monoclonal antibody , pregnancy
We studied the red cell antigens present on K562 human leukemia cells before and after induction of hemoglobin synthesis by hemin. The fetal antigens i, IF, and IT were detected on uninduced cells. While expression of both i and IT antigens increased after hemin induction, expression of IT was closely related to fetal hemoglobin synthesis as determined in experiments in which the induction was reversed. The EnaFR, NVg, and T antigens of glycophorin A were also present on uninduced cells. In contrast, the M and Pra antigens of glycophorin A, the Kell system antigens, and the P1 antigen became detectable only after hemin induction. Antigens of other major red cell systems were not detected.