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The importance of warming at 37 degrees C for removal of leukocytes and platelets from reconstituted red cells
Author(s) -
Shimizu T,
Noda Y,
Furuta A,
Morishima Y,
Goto S,
Hasegawa I,
Fukuda T
Publication year - 1984
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1984.24484275571.x
Subject(s) - platelet , centrifugation , buffy coat , incubation , suspension (topology) , chemistry , packed red blood cells , suspension culture , chromatography , microbiology and biotechnology , andrology , biology , biochemistry , immunology , cell culture , medicine , blood transfusion , genetics , mathematics , homotopy , pure mathematics
Red cells suspended in sodium phosphate buffer (R‐RBC) were incubated at 37 degrees C, and the distribution of the leukocytes and platelets in the suspension was compared to a similar suspension incubated at 22 degrees C. Following centrifugation, a more dense packing of the red cells was observed in warm R‐RBC, and less leukocytes and platelets remained in the red cells below the buffy coat, than was observed in R‐ RBC packed at room temperature. These experiments indicate that incubation at 37 degrees C prior to separation improves the quality of leukocyte‐ and platelet‐depleted red cells.