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Anti‐D prozone and membrane sulfhydryl modification
Author(s) -
Jameson J.T.,
Kleeman J.E.,
Masouredis S.P.,
Victoria E.J.
Publication year - 1984
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1984.24284173343.x
Subject(s) - thimerosal , chemistry , antigen , antibody , phenol , dissociation (chemistry) , biochemistry , immunology , biology , organic chemistry , medicine , dermatology
An IgG anti‐D prozone is produced by progressive inactivation of the D antigen following red cell exposure to increasing concentrations of thimerosal and phenol present as antibody excess is achieved. Partial inactivation of the D antigen by routinely added thimerosal, an organic mercurial, and phenol is associated with an unstable D antigen‐antibody complex resulting in an increased rate of dissociation of anti‐D and with a decreased reactivity of the cell‐bound anti‐D in the antiglobulin reaction. Complete D inactivation occurs with concentrations in excess of 0.43 microM thimerosal. If attributable to inactivation of a surface‐exposed sulfhydryl group, it suggests that less than 5 percent of these are involved in D‐antigen activity. The data do not exclude the possibility that D inactivation may result from alteration of sulfhydryl groups other than those exposed at the surface.