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Observations on the removal of microaggregates from stored blood by centrifugation and filtration through a standard 170 μm transfusion filter
Author(s) -
Linko K.
Publication year - 1983
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1983.23483276864.x
Subject(s) - centrifugation , filtration (mathematics) , microfiltration , chromatography , filter (signal processing) , chemistry , filter paper , membrane , biochemistry , mathematics , statistics , computer science , computer vision
The effect of centrifugation (5 minutes, 6900 ×g) on the amount and particle size of blood microaggregates was studied in order to assess whether centrifugation and filtration by a standard (170 μm) transfusion set filter could be used for aggregate removal instead of microfiltration. Aggregates were quantitated using a method based on nylon screens with graded pore sizes. At the beginning of storage, centrifugation increased the particle size of aggregates only slightly. In 7‐day‐old blood, aggregates capable of passing a 160 μm screen amounted to 308 ± 107 (SD) mg of debris protein per liter of blood before and 55 ± 26 (SD) mg of protein (n = 10) after centrifugation. In 14‐day‐old blood centrifugation increased the size of aggregates even more effectively: 311 ±81 (SD) before and 29 ± 14 (SD) mg of protein following centrifugation. Microaggregates can thus be removed effectively by the centrifugation and filtration method from blood stored for more than 1 week. A standard transfusion set filter can be used instead of a micropore filter.