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Enzyme‐linked immunosorbent microtiter assay for red cell serology
Author(s) -
Reppun T.,
McIlravy D.,
Sherman L. A.
Publication year - 1983
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1983.23483276863.x
Subject(s) - microtiter plate , reagent , red cell , enzyme , chemistry , chromatography , antibody , red blood cell , colorimetry , serology , biochemistry , microbiology and biotechnology , biology , immunology , medicine
Enzyme‐linked immunoabsorbent assays (EIA) permit quantitation and automation of reactions. Previous reports have also shown improved sensitivity for red cell antibodies. We have developed a microtiter technique for EIA, thus decreasing the amounts of reagents required. EDTA was used rather than NaOH to stop the enzyme reaction allowing more precise timing of reactions and improved quantitation of large batches of tests. As in prior reports, the sensitivity was slightly greater than the antiglobulin test, but occasional red cell antibodies failed to show specificity by EIA. The use of microtiter plates and EDTA decreased the steps and reagents required and has the potential for automation of at least the optical reading of such assays in the blood bank.