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Is a room‐temperature crossmatch necessary for the detection of ABO errors?
Author(s) -
Trudeau L. R.,
Judd W. J.,
Butch S. H.,
Oberman H. A.
Publication year - 1983
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1983.23383224903.x
Subject(s) - abo blood group system , saline , ionic strength , medicine , chemistry , immunology , aqueous solution
The detection of anti‐A and anti‐B isohemagglutinins by low‐ionic‐strength saline tests at 37°C and by the indirect antiglobulin technique, without an “immediate'spin” or room‐temperature phase, has been studied. Using such a procedure, all but one of 2746 patient blood samples reacted in accordance with ABO type when tested against A2 and B red cells. However, the discrepant sample also was nonreactive when tested by “immediate‐spin” technique against saline‐suspended A 2 red cells. Our findings indicate that compatibility tests performed at 37°C in low‐ionic‐strength saline are as sensitive as “immediate‐spin” tests with saline‐suspended red cells for the detection of ABO errors. Performing serologic tests for unexpected alloantibodies and donor‐recipient compatibility without an “immediate‐spin” or room‐temperature phase abbreviates pretransfusion testing and reduces the detection of clinically insignificant alloantibodies solely reactive at room temperature.