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Description and use of the CS‐3000 blood cell separator for single‐donor platelet collection
Author(s) -
Buchholz D. H.,
Porten J. H.,
Menitovea J. E.,
Rzad L.,
Bucheger R. R.,
Aster R. H.,
Lin A. T.,
Smith J.
Publication year - 1983
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1983.23383224893.x
Subject(s) - platelet , platelet concentrate , blood collection , medicine , surgery , separator (oil production) , zoology , chemistry , immunology , biology , emergency medicine , physics , thermodynamics
A new computer‐controlled seal less continuous flow blood cell separator has been developed to harvest platelets, plasma, or leukocytes and perform plasma exchange. One hundred seven platelet collection procedures were performed using an instrument prototype during developmental evaluation of a number of differently configured platelet collection chambers. An average of 3.6 (range 2.6‐5.5) × 10 11 platelets was collected during a 90‐minute period (blood withdrawl rate, 32 ml/minute) with the best chamber. Only a small number of other cells, from 0.1 to 0.2 times 10 9 white blood cells and 0.7 to 1.0 times 10 9 red cells was collected with the platelets. Total procedure time averaged 115 to 120 minutes and included 5 minutes to install the blood processing pathway, 10 minutes to prime the system under computer control, 5 minutes to return donor erythrocytes at the completion of the procedure, and 5 minutes to remove the processing set and resuspend the platelets. The average donor platelet count declined 48 times10 3 per μ (19%) by the completion of the procedure, and, with the exception of a decline in inorganic phosphorus from 3.4 to 2.5 mg per dl, no unexpected changes in serum chemistry levels were seen. Use of either of two larger separation chambers in a production version of the instrument permitted more rapid blood processing rates (≃45 ml/minute); an average of 4.2 (range 2.1–7.8) × 10 11 platelets was collected in 90 minutes using a larger chamber designed for platelet separation (n = 164). Platelets could also be collected using the chamber designed for white blood cell collection by omitting the use of hydroxyethyl starch and by selecting the platelet collection computer program rather than the white cell collection program. Using that chamber, an average of 4.3 ± 1.8 times 10 11 platelets was collected in 90 minutes (56% collection efficiency; n = 31). Leukocyte contamination of platelets was greater using the larger chambers. Infusion of autologous platelets labeled with 51 Cr demonstrated a 70 percent average recovery and a mean survival of 9.1 days (n = 10). Seven nonalloimmunized thrombocytopenic patients were transfused with platelets collected using the prototype cell separator and an average of 86 percent of the expected number of platelets was present in the circulation 2 hours after transfusion with 63 percent of the cells remaining in the circulation at 24 hours. Template bleeding times were corrected in six of seven recipients following transfusion, and hemostasis was achieved in three bleeding recipients. Therapeutically useful numbers of functionally effective platelets can be collected using this blood processing instrument.