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Quantitation of minor red cell populations using the single‐channel autoanalyzer
Author(s) -
Jeje M. O.,
Newton D.,
Blajchman M. A.
Publication year - 1983
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1983.23283172856.x
Subject(s) - autoanalyzer , population , red cell , antibody , chromatography , incubation period , chemistry , incubation , immunology , biology , biochemistry , medicine , environmental health
An automated hemagglutination technique has been applied to the quantitation of the size of minor red cell populations. By choosing an antibody to an antigen present only on the minor population, it was possible to estimate the size of the minor population by measuring the antibody following incubation with the red cell mixture. The size was directly proportional to the amount of antibody consumed and was calculated from a regression plot established from standard mixtures. With this method, it was possible to detect minor red cell populations as dilute as 1:5000.