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A technetium‐99m red cell survival technique for in vivo compatibility testing
Author(s) -
Holt J. T.,
Spitalnik S. L.,
McMican A. E.,
Wilson G.,
Blumberg N.
Publication year - 1983
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1983.23283172854.x
Subject(s) - in vivo , technetium 99m , heterologous , technetium , red cell , pyrophosphate , in vitro , chemistry , red blood cell , nuclear medicine , radiochemistry , medicine , scintigraphy , biochemistry , biology , microbiology and biotechnology , gene , enzyme
A technique for labeling red cells with Technetium‐99m was developed using in vitro reduction by stannous pyrophosphate. The use of Technetium‐99m and Chromium‐51 enabled simultaneous determination of 60‐minute autologous and heterologous red cell survival using small aliquots. In vivo pretransfusion red cell compatibility in a patient with a panagglutinin was identical to that with 51 Cr‐labeled cells. Simultaneous testing of two heterologous donor units or testing multiple units in the course of a few days was also feasible. In nine studies (one patient and six healthy controls) no significant elution of the 99m Tc was observed over 1 hour.