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Studies on the blood of an Mi v /M k proposita and her family
Author(s) -
Judd W. J.,
Geisland J. R.,
Issitt P. D.,
Wilkinson S. L.,
Anstee D. J.,
Shin C.,
Glidden H.
Publication year - 1983
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1983.23183147301.x
Subject(s) - heterozygote advantage , microbiology and biotechnology , gene , biology , antigen , red cell , genetics , immunology , medicine , allele
An individual (J‐1) was shown to be heterozygous for the Mi v and M k genes. Her red cells typed as M+(weak), N‐, S‐, s+(strong), U+, Hil+, Wr(a‐b‐), En(a+weak). Poly‐acrylamide gel electrophoresis analysis of her red cell membranes revealed absence of PAS‐staining bands corresponding to normal MN and Ss sialoglycoprotein (SGP), and presence of a hybrid MNSs SGP [(α ‐ δ)Mi v ] similar but not identical to that reported for an Mi v homozygote. However, J‐1 cannot be homozygous for Mi v since the red cells of two of her children are Hil‐ and s‐, carry only a single dose of M antigen, and have a sialic acid content that is consistent with the presumption that they are M k heterozygotes. J‐I's hybrid MNSs SGP is considered to be gene‐fusion product resulting from unequal crossover between a normal α M and δ gene, and her red cells lack that portion of the En a antigen that is resistant to ficin. Her hybrid MNSs SGP differs, therefore, from that reported for the Mi v homozygote, which probably arose from unequal crossover between α v and δ genes. Further, the red cells of the Mi v homozygote carry the ficin‐resistant En a determinant