Premium
Serologic comparison of a monoclonal anti‐C3d with commercial antiglobulin reagents
Author(s) -
Barker J.M.
Publication year - 1982
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1982.22683068614.x
Subject(s) - monoclonal antibody , monoclonal , in vivo , serology , chemistry , immunology , in vitro , medicine , microbiology and biotechnology , antibody , biology , biochemistry
The serologic reactivity of a monoclonal anti‐C3d and commercial “monospecific” anti‐C3 and polyspecific antiglobulin reagents was compared. The testing consisted of a direct antiglobulin test performed on donor and patient cells and on in vivo and in vitro complement‐ coated cells; an indirect antiglobulin test was performed with low‐ ionic‐strength solution crossmatches. The monoclonal anti‐C3d demonstrated reactivity very similar to that of the commercial heterogeneous rabbit anti‐C3d; it gave relatively few weak “false positive” reactions with normal donor and patient red blood cells, and comparably strong reactions with in vitro and in vivo C3d‐coated red blood cells. The results suggest a promising future for monoclonal antiglobulin reagents.