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A study of granulocyte cytotoxins and detection of granulocyte allospecific antigens
Author(s) -
Kořínková P.,
Vorlíček J.,
Májský A.
Publication year - 1982
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1982.22583017462.x
Subject(s) - granulocyte , titer , immunology , antibody , antigen , absorption (acoustics) , microbiology and biotechnology , biology , chemistry , materials science , composite material
Granulocytes were isolated b overlaying leukocyte‐rich plasma discontinuous density gradients. The cell suspensions were 95 percent granulocytes. Sera of 383 polytransfused patients and 521 multiparous women were examined for lymphocytotoxins, granulocytotoxins, and granulocytoagglutinins. On the basis of these tests, sera were identified which contained only granulocytotoxins. These were further identified with more precision as to their titers and antibody absorption; reactivity with granulocytes, B, and T lymphocytes; and K 562 cell line. On the basis of calculated correlation coefficients, nine sera were selected which determined three granulocyte specificities having genotype frequencies consistent with the Hardy‐Weinberg equilibrium.