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Antibody elution from red blood cells by chloroform and trichloroethylene
Author(s) -
Massuet L.,
Martin C.,
Ribera A.,
Argelagues E.,
DuranSuarez J.R.,
Triginer J.
Publication year - 1982
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1982.22583017457.x
Subject(s) - elution , trichloroethylene , chromatography , chemistry , chloroform , antibody , stroma , immunology , medicine , environmental chemistry , immunohistochemistry
Antibody elution by chloroform and trichloroethylene was compared with the Rubin ether method modified by Hughes‐Jones for IgG antibodies and the Landsteiner and Miller heat method for IgM antibodies. After washing the cells, the chloroform and trichloroethylene method was done at 37° C for 10 minutes. With this technique the eluate remained on the upper layer above the red blood cell stroma layer, while with Rubin's method the eluate remained below the cell stroma. The reported technique was as effective as the Landsteiner and Miller heat method for IgM antibodies, and a double volume of eluate was obtained.