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Comparison of two methods for concentrating stem cells for cryopreservation and transplantation
Author(s) -
Ma D. D. F.,
Biggs J. C.
Publication year - 1982
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1982.22382224944.x
Subject(s) - centrifugation , cryopreservation , bone marrow , stem cell , myeloid cells , dextran , transplantation , chemistry , andrology , myeloid , differential centrifugation , immunology , biology , chromatography , microbiology and biotechnology , medicine , surgery , embryo
Dextran sedimentation with centrifugation and semicontinuous flow centrifugation was used to concentrate marrow stem cells from collections of human bone marrow. With the dextran sedimentation method, recovery of the myeloid stem cells was 92 percent in 35 percent of the original volume, but contamination by mature cells was high. In the semicontinuous flow centrifugation method the recovery of myeloid stem cells was 82 percent in 15 percent of the original volume. Contamination with platelets, erythrocytes and neutrophils was reduced to 20.4, 29.6, and 38.6 percent respectively of the original values. It was concluded that semicontinuous flow centrifugation provides a comparatively convenient and efficient method for concentrating large collections of human bone marrow cells in a closed system.