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Two Examples of Antibodies Dependent upon the Presence of Inosine
Author(s) -
VengelenTyler V.,
Dearolf S. J.,
Haley S.
Publication year - 1981
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1981.21381201804.x
Subject(s) - antibody , inosine , diluent , red blood cell , hemagglutination , reagent , preservative , chemistry , titer , red cell , immunology , chromatography , microbiology and biotechnology , medicine , biology , biochemistry , nuclear chemistry , enzyme , food science
Two sera were found to react with red blood cells suspended in diluents containing inosine. The antibody in both cases was IgM in nature, of a low titer, and optimally reactive at temperatures of 22 and 4 C. The antibody was removed from the sera when absorbed with red blood cells suspended in inosine. Washing reagent red blood cells free of the preservative solutions prevented hemagglutination. Although these antibodies may mimic a high‐frequency antibody, they may be easily identified by washing the commercially prepared red blood cells free of the diluent solutions. Individuals demonstrating antibodies to components of the red blood cell diluent are relatively rare, and routine washing of commercially prepared red blood cells is not recommended.