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False‐Positive DAT Caused by Variables in Sample Procurement
Author(s) -
Grindon A. J.,
Wilson M. J.
Publication year - 1981
Publication title -
transfusion
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.045
H-Index - 132
eISSN - 1537-2995
pISSN - 0041-1132
DOI - 10.1046/j.1537-2995.1981.21381201803.x
Subject(s) - sample (material) , medicine , volume (thermodynamics) , chromatography , chemistry , physics , quantum mechanics
A positive DAT may occur when blood samples are drawn from an intravenous line and are a function of 1) drawing the sample from a line containing 5% or 10% dextrose rather than dextrose with lactated Ringer's; 2) using a large bore rather than a small bore needle; 3) using a clot rather than anticoagulated sample for the DAT; and 4) drawing a smaller volume of blood (reactions strongest at a sample size of 0.5 ml).